Nuclear receptor coactivator-1 (NCOA-1) as a member of Steroid receptor coactivator (SRC) family can interact with some DNA-bound nuclear receptors and enhance their transcriptional activation function, which is physiologically and pathologically important for animals and human. Much work has been done on the gene in human and mouse, but few in pig. Here we cloned and sequenced full length cDNA of NCOA-1 gene in pig. The putative protein includes 1440 amino acids. Sequences alignment showed that pig NCOA-1 gene cDNA sequence has identity of 93.73% with human and mouse, and putative pig NCOA-1 protein has identity of 94.99% with human, mouse and rat. The motifs, LXXLL, which have been demonstrated to be important for NCOA-1 mediating ligand-dependent and direct interaction with nuclear receptor, were conserved in pig.