Abstract
The Xenopus oocyte is a widely used system for protein expression. Investigators have had the choice between two different techniques: injection into the cytoplasm of in vitro transcribed complementary RNA (cRNA) or injection into the nucleus of complementary DNA (cDNA). We report on a third expression technique that is based on the combined injection of cDNA and purified T7 RNA polymerase directly into the cytoplasm of oocytes.
MeSH terms
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Animals
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Bacteriophage T7 / enzymology
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DNA-Directed RNA Polymerases* / chemistry
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DNA-Directed RNA Polymerases* / isolation & purification
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Female
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Microinjections* / instrumentation
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Microinjections* / methods
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Nanotechnology / instrumentation
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Nanotechnology / methods
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Oocytes / metabolism*
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Viral Proteins* / chemistry
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Viral Proteins* / isolation & purification
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Xenopus laevis
Substances
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Viral Proteins
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bacteriophage T7 RNA polymerase
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DNA-Directed RNA Polymerases