Unusual type of TLS/FUS-ERG chimeric transcript in a pediatric acute myelocytic leukemia with 47,XX,+10,t(16;21)(p11;q22)

Cancer Genet Cytogenet. 2006 Jun;167(2):172-6. doi: 10.1016/j.cancergencyto.2006.01.003.

Abstract

We report on a case of pediatric acute myelocytic leukemia showing 47,XX,+10,t(16;21)(p11;q22) that resulted in an unusual TLS/FUS-ERG chimeric transcript. The leukemic cells showed erythrophagocytosis, positive reactions for myeloperoxidase and Sudan black B stains, and negative reactions for periodic acid-Schiff and alpha-naphtyl butyrate esterase stains as well as expression of myeloid antigens. We also confirmed a very rare type of TLS/FUS-ERG chimeric transcript by fusion of the 5' part of the TLS/FUS gene in chromosome 16p11 and the 3' part of the ERG gene in chromosome 21q22 using reverse-transcriptase polymerase chain reaction and direct sequencing. After achieving a complete remission with two cycles of induction chemotherapy, the patient received an umbilical cord blood transplantation.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bone Marrow Cells / cytology
  • Child, Preschool
  • Chromosomes, Human, Pair 10
  • Chromosomes, Human, Pair 16* / ultrastructure
  • Chromosomes, Human, Pair 21* / ultrastructure
  • Female
  • Humans
  • Leukemia, Myeloid, Acute / genetics*
  • Leukemia, Myeloid, Acute / pathology
  • Oncogene Proteins, Fusion / genetics*
  • RNA, Messenger / analysis
  • RNA-Binding Protein FUS / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Translocation, Genetic*
  • Trisomy

Substances

  • Oncogene Proteins, Fusion
  • RNA, Messenger
  • RNA-Binding Protein FUS
  • TLS-ERG fusion protein, human