Dimethyl-acetamide as a cryoprotectant for rainbow trout spermatozoa

M A McNiven; R K Gallant; G F Richardson

doi:10.1016/0093-691x(93)90362-9

Dimethyl-acetamide as a cryoprotectant for rainbow trout spermatozoa

Theriogenology. 1993 Nov;40(5):943-8. doi: 10.1016/0093-691x(93)90362-9.

Authors

M A McNiven¹, R K Gallant, G F Richardson

Affiliation

¹ Department of Health Management Atlantic Veterinary College University of Prince Edward Island Charlottetown, P.E.I., Canada, C1A 4P3.

PMID: 16727376
DOI: 10.1016/0093-691x(93)90362-9

Abstract

Dimethyl-acetamide was evaluated as a cryoprotectant for rainbow trout (Oncorhynchus mykiss ) semen on the basis of motility, percentage of dead spermatozoa as determined by fluorometry, and fertility of frozen-thawed spermatozoa. Dimethyl-acetamide performed significantly better (P<0.05) than the conventional cryoprotectant, dimethyl sulfoxide, by all evaluation methods. An extender comprised of 0.137 M NaCl, 0.011 M KCl, 0.004 M Na(2)HPO(4) 7H(2)O, 7.5 g/l L-alpha-lecithin and 10 % DMA showed promise for cryopreserving rainbow trout spermatozoa. Future evaluation of new extenders should be carried out by several in vitro techniques and fertility measurements to present a complete assessment of an extender's ability to cryopreserve sperm cells.