Objective: This paper aimed at improving existing breeds of Phellodendron chinense and enriching the resources of heredity breeding.
Method: The polyploid of P. chinense was obtained using calli, seeds and buds, which were treated with colchicines solution.
Result: The polyploid of P. chinense was obtained using calli with stem treated with 0.2% colchicines medium, the inducing rate reached 12.45%. Compared with the morphology and chromosomal number the polyploid induction was proved to be effective.
Conclusion: It was an effective biotechnological way to use colchicines to induce polyploid on the different explants of P. chinense.