U1 and U2 small nuclear RNA genetic linkage: a novel molecular tool for identification of six sole species (Soleidae, Pleuronectiformes)

Manuel Manchado; Laureana Rebordinos; Carlos Infante

doi:10.1021/jf0530594

U1 and U2 small nuclear RNA genetic linkage: a novel molecular tool for identification of six sole species (Soleidae, Pleuronectiformes)

J Agric Food Chem. 2006 May 31;54(11):3765-7. doi: 10.1021/jf0530594.

Authors

Manuel Manchado¹, Laureana Rebordinos, Carlos Infante

Affiliation

¹ CIFPA "El Toruño", I.F.A.P.A. C.I.C.E. (Junta de Andalucía). Camino tiro de pichón s/n, 11500 El Puerto de Santa María, Cadiz, Spain. manuel.manchado.ext@juntadeandalucia.es

PMID: 16719493
DOI: 10.1021/jf0530594

Abstract

We evaluated the usefulness of a genetic linkage between the U1 and U2 small nuclear RNAs for species identification. Six soles belonging to the genera Solea, Dicologlossa, and Microchirus were studied. A simple methodology based on two single PCRs is described. Reproducible band profiles were generated for all samples. This rapid and discriminatory molecular method is highly promising for determining the authenticity of sole fillets in the food industry.

MeSH terms

Animals
DNA / analysis
DNA / chemistry
Flatfishes / classification*
Flatfishes / genetics*
Food Industry
Genetic Linkage*
Polymerase Chain Reaction
RNA, Small Nuclear / genetics*
Reproducibility of Results

Substances

RNA, Small Nuclear
U1 small nuclear RNA
U2 small nuclear RNA
DNA