Degradation of PrP(Sc) by keratinolytic protease from Nocardiopsis sp. TOA-1

Shinji Mitsuiki; Zhao Hui; Daishi Matsumoto; Masashi Sakai; Yasushi Moriyama; Kensuke Furukawa; Hiroaki Kanouchi; Tatsuzo Oka

doi:10.1271/bbb.70.1246

Degradation of PrP(Sc) by keratinolytic protease from Nocardiopsis sp. TOA-1

Biosci Biotechnol Biochem. 2006 May;70(5):1246-8. doi: 10.1271/bbb.70.1246.

Authors

Shinji Mitsuiki¹, Zhao Hui, Daishi Matsumoto, Masashi Sakai, Yasushi Moriyama, Kensuke Furukawa, Hiroaki Kanouchi, Tatsuzo Oka

Affiliation

¹ Department of Applied Chemistry and Biochemistry, Kyushu Sangyo University, Fukuoka, Japan.

PMID: 16717429
DOI: 10.1271/bbb.70.1246

Abstract

A keratinolytic alkaline proteae (NAPase) from Nocardiopsis sp. TOA-1 degraded a scrapie prion without any chemical or physical treatment. Optimal temperature and pH were 60 degrees C and above pH 10.0. The scrapie prion was completely degraded within 3 min under optimal conditions.

MeSH terms

Actinomycetales / enzymology*
Animals
Biodegradation, Environmental
Cricetinae
Hydrogen-Ion Concentration
Male
Peptide Hydrolases / metabolism*
PrPSc Proteins / metabolism*
Temperature

Substances

PrPSc Proteins
Peptide Hydrolases
keratinase