Amplification and diversity analysis of ketosynthase domains of putative polyketide synthase genes in Aspergillus ochraceus and Aspergillus carbonarius producers of ochratoxin A

Ali Atoui; Huy Phong Dao; Florence Mathieu; Ahmed Lebrihi

doi:10.1002/mnfr.200500165

Amplification and diversity analysis of ketosynthase domains of putative polyketide synthase genes in Aspergillus ochraceus and Aspergillus carbonarius producers of ochratoxin A

Mol Nutr Food Res. 2006 May;50(6):488-93. doi: 10.1002/mnfr.200500165.

Authors

Ali Atoui¹, Huy Phong Dao, Florence Mathieu, Ahmed Lebrihi

Affiliation

¹ Département Bioprocédés et Systèmes Microbiens, Laboratoire de Génie Chimique UMR5503 (CNRS/INPT/UPS), Ecole Nationale Supérieure Agronomique de Toulouse, Institut National Polytechnique de Toulouse, France.

PMID: 16715542
DOI: 10.1002/mnfr.200500165

Abstract

The diversity of polyketide synthase (PKS) genes in Aspergillus ochraceus NRRL 3174 and Aspergillus carbonarius 2Mu134 has been investigated using different primer pairs previously developed for the ketosynthase (KS) domain of fungal PKSs. Nine different KS domain sequences in A. ochraceus NRRL 3174 as well as five different KS domain sequences in A. carbonarius 2Mu134 have been identified. The identified KS fragments were distributed in five different clusters on the phylogenetic tree, indicating that they most probably represent PKSs responsible for different functions.

MeSH terms

Amino Acid Sequence
Aspergillus / enzymology*
Aspergillus / genetics
Aspergillus ochraceus / enzymology*
Aspergillus ochraceus / genetics
Base Sequence
Chromatography, High Pressure Liquid
DNA, Bacterial / analysis
DNA, Bacterial / chemistry
DNA, Bacterial / isolation & purification
Molecular Sequence Data
Penicillic Acid / metabolism
Phylogeny
Polyketide Synthases / chemistry*
Polyketide Synthases / genetics*
Polymerase Chain Reaction*

Substances

DNA, Bacterial
Polyketide Synthases
Penicillic Acid