Identification of conjugated linoleic acid elongation and beta-oxidation products by coupled silver-ion HPLC APPI-MS

André Müller; Markus Mickel; Roland Geyer; Robert Ringseis; Klaus Eder; Hans Steinhart

doi:10.1016/j.jchromb.2006.04.012

Identification of conjugated linoleic acid elongation and beta-oxidation products by coupled silver-ion HPLC APPI-MS

J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Jun 6;837(1-2):147-52. doi: 10.1016/j.jchromb.2006.04.012. Epub 2006 May 19.

Authors

André Müller¹, Markus Mickel, Roland Geyer, Robert Ringseis, Klaus Eder, Hans Steinhart

Affiliation

¹ Institute of Biochemistry and Food Chemistry, Department of Food Chemistry, University of Hamburg, Grindelallee 117, 20146 Hamburg, Germany.

PMID: 16713405
DOI: 10.1016/j.jchromb.2006.04.012

Abstract

Atmospheric pressure photoionisation (APPI) was used in combination with silver-ion (Ag(+))-HPLC for detection of (conjugated) fatty acid methyl esters (FAME) by tandem-mass spectrometry. APPI-MS of methyl esters of conjugated linoleic acid showed an increase in signal-to-noise ratio by a factor of 40 compared to atmospheric pressure chemical ionization in the positive mode. It was possible to identify double bond position, configuration and chain length of FAME based on chromatographic separation and mass detection. The developed LC-MS method is useful for the analysis of CLA elongation and beta-oxidation products, especially with trans,trans-configuration, which are difficult to analyze by conventional GC-MS techniques.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Atmospheric Pressure
Chromatography, High Pressure Liquid / methods*
Linoleic Acid / chemistry*
Mass Spectrometry / methods*
Oxidation-Reduction
Sensitivity and Specificity
Silver / chemistry*

Substances

Silver
Linoleic Acid