Expression of an active foot-and-mouth disease virus RNA polymerase in Escherichia coli

Braz J Med Biol Res. 1991;24(4):359-63.

Abstract

The expression of a native form of the foot-and-mouth disease virus RNA polymerase was obtained. Two oligonucleotides of 66 base pairs were used to rebuild the 5' end of the gene and to introduce the first methionine codon. The expression of the active polymerase in E. coli was achieved by inserting the gene before the tac promoter of the pKK223-3 plasmid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Aphthovirus / enzymology
  • Aphthovirus / genetics*
  • Base Sequence
  • Cloning, Molecular
  • Codon / genetics
  • DNA-Directed RNA Polymerases / genetics*
  • DNA-Directed RNA Polymerases / isolation & purification
  • Electrophoresis, Agar Gel
  • Escherichia coli / genetics*
  • Molecular Sequence Data
  • Oligonucleotides / chemistry
  • Plasmids
  • Transfection

Substances

  • Codon
  • Oligonucleotides
  • DNA-Directed RNA Polymerases