DNA sequence analysis of a small cryptic plasmid from Lactococcus lactis subsp. lactis M14

A R Raha; W Y Hooi; N S Mariana; S Radu; N R S Varma; K Yusoff

doi:10.1016/j.plasmid.2006.03.003

DNA sequence analysis of a small cryptic plasmid from Lactococcus lactis subsp. lactis M14

Plasmid. 2006 Jul;56(1):53-61. doi: 10.1016/j.plasmid.2006.03.003. Epub 2006 May 3.

Authors

A R Raha¹, W Y Hooi, N S Mariana, S Radu, N R S Varma, K Yusoff

Affiliation

¹ Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. raha@biotech.upm.edu.my

PMID: 16675013
DOI: 10.1016/j.plasmid.2006.03.003

Abstract

A small plasmid designated pAR141 was isolated from Lactococcus lactis subsp. lactis M14 and its complete 1,594 base pair nucleotide sequence was determined. Analysis of the sequence indicated that this plasmid does not carry any industrially important determinants besides the elements involved in plasmid replication and control. The transcriptional repressor CopG and replication initiation protein RepB appeared as a single operon. A small countertranscribed RNA (ctRNA) coding region was found between the copG and repB genes. The double strand origin (dso) and single strand origin (sso) of rolling circle replicating (RCR) plasmids were also identified in pAR141, suggesting that this plasmid replicates by rolling circle (RC) mode. This observation was supported by S1 nuclease and Southern hybridization analyses.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Base Sequence
Blotting, Southern
Cloning, Molecular
DNA Restriction Enzymes / pharmacology
DNA, Single-Stranded / chemistry
Lactococcus / metabolism
Lactococcus lactis / genetics*
Molecular Sequence Data
Open Reading Frames
Plasmids / metabolism*
Sequence Analysis, DNA*
Sequence Homology, Nucleic Acid

Substances

DNA, Single-Stranded
DNA Restriction Enzymes