Isolation of Gln- mutants through transposon, Tn917, mutagenesis in Bacillus brevis

H S Mishra; D P Mishra; G K Garg

Isolation of Gln- mutants through transposon, Tn917, mutagenesis in Bacillus brevis

Indian J Biochem Biophys. 1991 Oct-Dec;28(5-6):472-5.

Authors

H S Mishra¹, D P Mishra, G K Garg

Affiliation

¹ Department of Biochemistry, G.B. Pant University of Agriculture & Technology, Pantnagar.

PMID: 1667395

Abstract

Transposon, Tn917, carried on pTV1 plasmid has been used successfully to mutagenise Bacillus brevis. The transposon showed preference for insertion at an "aro" site. A second insertional event after elimination of the preferred site with ethidium bromide/acridine orange treatment has permitted isolation of Gln- mutants in B. brevis.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Bacillus / genetics*
DNA Transposable Elements
Glutamine / genetics
Mutagenesis, Insertional

Substances

DNA Transposable Elements
Glutamine