Development and validation of an oligonucleotide microarray for detection of multiple virulence and antimicrobial resistance genes in Escherichia coli

Guillaume Bruant; Christine Maynard; Sadjia Bekal; Isabelle Gaucher; Luke Masson; Roland Brousseau; Josée Harel

doi:10.1128/AEM.72.5.3780-3784.2006

Development and validation of an oligonucleotide microarray for detection of multiple virulence and antimicrobial resistance genes in Escherichia coli

Appl Environ Microbiol. 2006 May;72(5):3780-4. doi: 10.1128/AEM.72.5.3780-3784.2006.

Authors

Guillaume Bruant¹, Christine Maynard, Sadjia Bekal, Isabelle Gaucher, Luke Masson, Roland Brousseau, Josée Harel

Affiliation

¹ Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de Médecine Vétérinaire, Université de Montréal, 3200 rue Sicotte, Saint-Hyacinthe, Québec J2S 7C6, Canada.

Abstract

An oligonucleotide microarray detecting 189 Escherichia coli virulence genes or markers and 30 antimicrobial resistance genes was designed and validated using DNA from known reference strains. This microarray was confirmed to be a powerful diagnostic tool for monitoring emerging E. coli pathotypes and antimicrobial resistance, as well as for environmental, epidemiological, and phylogenetic studies including the evaluation of genome plasticity.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Drug Resistance, Bacterial / genetics*
Escherichia coli / drug effects*
Escherichia coli / genetics
Escherichia coli / pathogenicity*
Escherichia coli Proteins / genetics
Escherichia coli Proteins / metabolism*
Gene Expression Profiling
Humans
Oligonucleotide Array Sequence Analysis / methods*
Virulence / genetics

Substances

Escherichia coli Proteins