Influence of sporulation medium composition on transcription of ger operons and the germination response of spores of Bacillus cereus ATCC 14579

Luc M Hornstra; Ynte P de Vries; Willem M de Vos; Tjakko Abee

doi:10.1128/AEM.72.5.3746-3749.2006

Influence of sporulation medium composition on transcription of ger operons and the germination response of spores of Bacillus cereus ATCC 14579

Appl Environ Microbiol. 2006 May;72(5):3746-9. doi: 10.1128/AEM.72.5.3746-3749.2006.

Authors

Luc M Hornstra¹, Ynte P de Vries, Willem M de Vos, Tjakko Abee

Affiliation

¹ Laboratory of Food Microbiology, Wageningen University, Bomenweg 2, 6703 HD Wageningen, The Netherlands.

Abstract

Bacillus cereus ATCC 14579 endospores were produced in Y1 medium, a nutrient-rich, chemically defined sporulation medium, and in modified G medium, containing low amounts of nutrients. The average transcription level of the seven ger operons per cell was 3.5 times higher in Y1 medium, and the spores grown in this medium showed an enhanced germination response.

MeSH terms

Bacillus cereus / genetics
Bacillus cereus / physiology*
Bacterial Proteins / genetics
Bacterial Proteins / metabolism*
Culture Media / chemistry*
Gene Expression Regulation, Bacterial*
Operon*
Spores, Bacterial / physiology
Transcription, Genetic

Substances

Bacterial Proteins
Culture Media