Abstract
We have developed a new DNA chip whose substrate has a unique minute columnar array structure made of plastic. The DNA chip exhibits ultrahigh sensitivity, up to 100-fold higher than that of reference DNA chips, which makes it possible to monitor gene expression profiles even with very small amounts of RNA (0.1-0.01 microg of total RNA) without amplification. Differential expression ratios obtained with the new DNA chip were validated against those obtained with quantitative real-time PCR assays. This novel microarray technology would be a powerful tool for monitoring gene expression profiles, especially for clinical diagnosis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Gene Expression Profiling / instrumentation
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Gene Expression Profiling / methods*
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Microscopy, Electron, Scanning
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Nucleic Acid Amplification Techniques / methods*
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Nucleic Acid Hybridization / methods
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Oligonucleotide Array Sequence Analysis / instrumentation
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Oligonucleotide Array Sequence Analysis / methods*
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Plasminogen Activators / genetics
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RNA / genetics
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RNA / metabolism*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Reproducibility of Results
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Reverse Transcriptase Polymerase Chain Reaction / methods
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Statistics, Nonparametric
Substances
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RNA, Messenger
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RNA
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Plasminogen Activators