Ultrasensitive DNA chip: gene expression profile analysis without RNA amplification

J Biochem. 2006 Apr;139(4):697-703. doi: 10.1093/jb/mvj086.

Abstract

We have developed a new DNA chip whose substrate has a unique minute columnar array structure made of plastic. The DNA chip exhibits ultrahigh sensitivity, up to 100-fold higher than that of reference DNA chips, which makes it possible to monitor gene expression profiles even with very small amounts of RNA (0.1-0.01 microg of total RNA) without amplification. Differential expression ratios obtained with the new DNA chip were validated against those obtained with quantitative real-time PCR assays. This novel microarray technology would be a powerful tool for monitoring gene expression profiles, especially for clinical diagnosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Gene Expression Profiling / instrumentation
  • Gene Expression Profiling / methods*
  • Microscopy, Electron, Scanning
  • Nucleic Acid Amplification Techniques / methods*
  • Nucleic Acid Hybridization / methods
  • Oligonucleotide Array Sequence Analysis / instrumentation
  • Oligonucleotide Array Sequence Analysis / methods*
  • Plasminogen Activators / genetics
  • RNA / genetics
  • RNA / metabolism*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Reproducibility of Results
  • Reverse Transcriptase Polymerase Chain Reaction / methods
  • Statistics, Nonparametric

Substances

  • RNA, Messenger
  • RNA
  • Plasminogen Activators