Preparation of chlamydomonas chloroplasts for the in vitro import of polypeptide precursors

M Goldschmidt-Clermont; P Malnoë; J D Rochaix

doi:10.1104/pp.89.1.15

Preparation of chlamydomonas chloroplasts for the in vitro import of polypeptide precursors

Plant Physiol. 1989 Jan;89(1):15-8. doi: 10.1104/pp.89.1.15.

Authors

M Goldschmidt-Clermont¹, P Malnoë, J D Rochaix

Affiliation

¹ Department of Molecular Biology, University of Geneva, Sciences II, 30 quai E. Ansermet, 1211 Genève 4, Switzerland.

Abstract

To study the import of polypeptide precursors we have adapted and compared two procedures for the isolation of competent chloroplasts from the green unicellular alga, Chlamydomonas reinhardtii: silicasol gradient centrifugation and elutriation. The chloroplasts actively import the precursor of the small subunit of ribulose bisphosphate carboxylase-oxygenase in vitro.