Overexpression of EIF3S3 promotes cancer cell growth

Prostate. 2006 Aug 1;66(11):1144-50. doi: 10.1002/pros.20452.

Abstract

Background: Amplification and overexpression of EIF3S3 gene has been demonstrated in breast and prostate cancer. Here, our goal was to study the effect of EIF3S3 on cell growth.

Methods: The effect of EIF3S3 on growth of NIH 3T3 murine fibroblasts as well as breast (SK-Br-3 and ZR-75-1) and prostate (PC-3 and LNCaP) cancer cell lines was examined by using transfection with inducible pTet-Off system and siRNAs.

Results: NIH 3T3 cells with overexpression of EIF3S3 grew significantly faster than cells transfected with empty vector and survived longer when grown in soft agar. The EIF3S3 overexpression was associated with increased fraction of cells in S-phase and with phosphorylation of retinoblastoma (Rb) protein. siRNA treatment inhibited significantly (P = 0.0022) the growth of all breast and prostate cancer cell lines studied.

Conclusions: The results suggest that EIF3S3 regulates cell growth and viability, and that overexpression of the gene may provide growth advantage to the cancer cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis
  • Cell Division*
  • Cell Line, Tumor
  • Eukaryotic Initiation Factor-3 / genetics*
  • Gene Expression*
  • Humans
  • Male
  • Mice
  • NIH 3T3 Cells
  • Phosphorylation
  • Prostatic Neoplasms / genetics*
  • Prostatic Neoplasms / pathology*
  • RNA, Small Interfering / genetics
  • Retinoblastoma Protein / metabolism
  • S Phase
  • Transfection

Substances

  • Eukaryotic Initiation Factor-3
  • RNA, Small Interfering
  • Retinoblastoma Protein