Investigations were carried out to assess the generation and disposal of biomedical waste in the various medical establishments in the urban and rural areas of the U.T. Chandigarh. It was found that there were 474 medical establishments in the U.T., Chandigarh including Nursing Homes, Clinics, Dispensaries, Pathological labs., Hospitals, Veterinary Institutions and Animal houses. The total quantity of bio-medical waste generated in Chandigarh is 811.35 kg/day and the rate of generation of bio-medical waste varies from 0.06 kg/day/bed to 0.25 kg/day/bed. Though the major hospitals are equipped with incinerators, proper bio-medical waste management system is yet to be implemented. The medical establishments in the rural area and smaller ones in the urban area dispose off their bio-medical waste along with municipal solid waste and no waste management system exists. It is recommended that an integrated waste management plan using the three incinerators installed at the major hospitals can safely dispose off the total bio-medical waste generated in the city.