Purification of ferredoxin-NADP+ reductase, flavodoxin and ferredoxin from a single batch of the cyanobacterium Anabaena PCC 7119

Prep Biochem. 1991;21(4):191-204. doi: 10.1080/10826069108018571.

Abstract

Methods are described for the simultaneous isolation of ferredoxin-NADP+ reductase, ferredoxin and flavodoxin from large quantities of the cyanobacterium Anabaena PCC 7119 allowing the use of a single batch of cells. The ultraviolet-visible spectra and the extinction coefficients of ferredoxin-NADP+ reductase and ferredoxin were determined. The purification procedure also yields enriched fractions of phycobiliproteins and cytochrome c553.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anabaena / chemistry*
  • Anabaena / enzymology
  • Cell Division
  • Chromatography, DEAE-Cellulose
  • Cytochrome c Group / isolation & purification
  • Ferredoxin-NADP Reductase / chemistry
  • Ferredoxin-NADP Reductase / isolation & purification*
  • Ferredoxins / chemistry
  • Ferredoxins / isolation & purification*
  • Flavodoxin / chemistry
  • Flavodoxin / isolation & purification*
  • Light-Harvesting Protein Complexes
  • Plant Proteins / isolation & purification
  • Spectrophotometry, Ultraviolet

Substances

  • Cytochrome c Group
  • Ferredoxins
  • Flavodoxin
  • Light-Harvesting Protein Complexes
  • Plant Proteins
  • cytochrome c553
  • cytochrome C-552
  • Ferredoxin-NADP Reductase