Abstract
Methods are described for the simultaneous isolation of ferredoxin-NADP+ reductase, ferredoxin and flavodoxin from large quantities of the cyanobacterium Anabaena PCC 7119 allowing the use of a single batch of cells. The ultraviolet-visible spectra and the extinction coefficients of ferredoxin-NADP+ reductase and ferredoxin were determined. The purification procedure also yields enriched fractions of phycobiliproteins and cytochrome c553.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anabaena / chemistry*
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Anabaena / enzymology
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Cell Division
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Chromatography, DEAE-Cellulose
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Cytochrome c Group / isolation & purification
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Ferredoxin-NADP Reductase / chemistry
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Ferredoxin-NADP Reductase / isolation & purification*
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Ferredoxins / chemistry
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Ferredoxins / isolation & purification*
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Flavodoxin / chemistry
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Flavodoxin / isolation & purification*
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Light-Harvesting Protein Complexes
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Plant Proteins / isolation & purification
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Spectrophotometry, Ultraviolet
Substances
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Cytochrome c Group
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Ferredoxins
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Flavodoxin
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Light-Harvesting Protein Complexes
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Plant Proteins
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cytochrome c553
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cytochrome C-552
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Ferredoxin-NADP Reductase