Exposure to modeled microgravity induces metabolic idleness in malignant human MCF-7 and normal murine VSMC cells

Rita Coinu; Angela Chiaviello; Grazia Galleri; Flavia Franconi; Elvira Crescenzi; Giuseppe Palumbo

doi:10.1016/j.febslet.2006.03.078

Exposure to modeled microgravity induces metabolic idleness in malignant human MCF-7 and normal murine VSMC cells

FEBS Lett. 2006 May 1;580(10):2465-70. doi: 10.1016/j.febslet.2006.03.078. Epub 2006 Apr 7.

Authors

Rita Coinu¹, Angela Chiaviello, Grazia Galleri, Flavia Franconi, Elvira Crescenzi, Giuseppe Palumbo

Affiliation

¹ Dipartimento di Patologia e Biologia Cellulare e Molecolare/IEOS CNR, Università di Napoli Federico II, Via S. Pansini, 5, I-80131 Naples, Italy.

PMID: 16638572
DOI: 10.1016/j.febslet.2006.03.078

Abstract

We investigated the effect of modeled microgravity (MMG) on normal vascular smooth muscle cells (VSMC) and neoplastic human breast cancer cells (MCF-7). In both cell types, MMG induced partial arrest in G2M and increased p14-3-3, HSP70, HSP60 and p21 expression. Cells synchronized by 24h starvation reentered the normal cycle within 24h if released in complete medium and exposed to normal gravity, but not if exposed to MMG. Similarly, MMG prevented VSMC and MCF-7 cells from overcoming growth arrest and re-synthesizing DNA. This study shows that cells adjust their metabolic rate in response to MMG.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Breast Neoplasms / metabolism*
Breast Neoplasms / pathology
Cell Division
Cell Line, Tumor
Cell Proliferation
Cells, Cultured
Electrophoresis, Polyacrylamide Gel
Flow Cytometry
Humans
Mice
Muscle, Smooth, Vascular / cytology
Muscle, Smooth, Vascular / metabolism*
Weightlessness*