Accurate measurement of long-range heteronuclear coupling constants from undistorted multiplets of an enhanced CPMG-HSQMBC experiment

Katalin E Kövér; Gyula Batta; Krisztina Fehér

doi:10.1016/j.jmr.2006.03.015

Accurate measurement of long-range heteronuclear coupling constants from undistorted multiplets of an enhanced CPMG-HSQMBC experiment

J Magn Reson. 2006 Jul;181(1):89-97. doi: 10.1016/j.jmr.2006.03.015. Epub 2006 Apr 18.

Authors

Katalin E Kövér¹, Gyula Batta, Krisztina Fehér

Affiliation

¹ Department of Inorganic and Analytical Chemistry, University of Debrecen, Egyetem tér 1, H-4010 Debrecen, Hungary. kover@tigris.unideb.hu

PMID: 16621632
DOI: 10.1016/j.jmr.2006.03.015

Abstract

Here, we present a modified CPMG-HSQMBC experiment which is capable to reduce the detrimental phase twists in the "long-range" connectivity multiplets caused by proton-proton couplings. We demonstrate that concerted CPMG pulse trains applied on both nuclei in the starting CPMG-INEPT transfer step can considerably be improved by composite pi pulses that compensate for pulse imperfections and off-resonance effects. Experimental optimization of the interpulse delay within the CPMG cycle was found to be crucial in order to achieve the best possible "decoupling" of homonuclear coupling modulation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Glycopeptides / chemistry*
Molecular Structure
Nitrogen Isotopes
Nuclear Magnetic Resonance, Biomolecular / methods*

Substances

Glycopeptides
Nitrogen Isotopes
eremomycin