Abstract
To investigate the use of embryonic stem cells as biosensor elements, mouse embryoid bodies were cultured on the surface of the light-addressable potentiometric sensor and induce to in vitro differentiate into cardiomyocytes and neurons. Extracellular potentials of the cells were recorded by sensor, to detect stem cells potential applications in drugs screening. The experimental results show that known cardiac stimulants (isoproterenol) and relaxants (carbamylcholine) have characteristic effects on the cardiomyocytes in terms of the changes of beat frequency, amplitude and duration. Thus, the embryonic stem cells potentially represent a renewable cell source for the cell-based biosensors.
Publication types
-
Evaluation Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Action Potentials / drug effects
-
Action Potentials / physiology*
-
Animals
-
Biological Assay / instrumentation*
-
Biological Assay / methods
-
Biosensing Techniques / instrumentation*
-
Biosensing Techniques / methods
-
Carbachol / administration & dosage
-
Cardiotonic Agents / administration & dosage*
-
Cell Culture Techniques / instrumentation
-
Cell Culture Techniques / methods
-
Cell Line
-
Dose-Response Relationship, Drug
-
Drug Evaluation, Preclinical / instrumentation*
-
Drug Evaluation, Preclinical / methods
-
Electrochemistry / instrumentation
-
Electrochemistry / methods
-
Embryonic Stem Cells / cytology
-
Embryonic Stem Cells / drug effects
-
Embryonic Stem Cells / physiology*
-
Equipment Design
-
Equipment Failure Analysis
-
Isoproterenol / administration & dosage
-
Mice
-
Microelectrodes
-
Myocytes, Cardiac / cytology
-
Myocytes, Cardiac / drug effects
-
Myocytes, Cardiac / physiology*
Substances
-
Cardiotonic Agents
-
Carbachol
-
Isoproterenol