Efficient generation of insect-based cell-free translation extracts active in glycosylation and signal sequence processing

Federico Katzen; Wieslaw Kudlicki

doi:10.1016/j.jbiotec.2006.03.002

Efficient generation of insect-based cell-free translation extracts active in glycosylation and signal sequence processing

J Biotechnol. 2006 Sep 1;125(2):194-7. doi: 10.1016/j.jbiotec.2006.03.002. Epub 2006 Apr 18.

Authors

Federico Katzen¹, Wieslaw Kudlicki

Affiliation

¹ Invitrogen Corp, Carlsbad, CA 92008, USA.

PMID: 16621082
DOI: 10.1016/j.jbiotec.2006.03.002

Abstract

A novel method for generation of insect-based cell-free translation extracts is presented. The protocol can be completed in less than an hour, and the resulting extracts are extremely proficient in N-linked glycosylation and signal sequence processing. No specialized equipment other than that usually present in an ordinary biochemistry laboratory is required. The novel approach dramatically reduces cost and time while rendering enhanced lysates compared to previously published strategies.

MeSH terms

Animals
Biotechnology / methods
Cell Extracts / chemistry
Cell Line
Cell-Free System / metabolism
Glycosylation
Protein Biosynthesis / genetics*
Protein Processing, Post-Translational*
Protein Sorting Signals / genetics*
Reproducibility of Results
Spodoptera / cytology
Spodoptera / metabolism*

Substances

Cell Extracts
Protein Sorting Signals