Objective: To prepare and purify polyclonal antibody against chymopapain, and to make a foundation for establishing an immunossay for chymopapain.
Methods: New Zealand rabbit was immunized with chymopapain. Antiserum was purified by Protein A and analyzed by ELISA.
Results: The titer of the antiserum obtained in this experiment by ELISA was up to 1:380000 and the purity was proved to be high by SDS-PAGE.