Abstract
Over-production of human soluble B lymphocyte stimulator (hsBLyS) was carried out with four different fed-batch culture strategies using lactose as inducer, instead of IPTG, in a fed-batch culture of Escherichia coli. As lactose acted as both inducer and carbon source, the best and simplest culture strategy was direct feeding of lactose after batch culture, thereby giving hsBLyS at 3.7 g l(-1) and a productivity of 0.11 g l(-1) h(-1).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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B-Cell Activating Factor / genetics*
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B-Cell Activating Factor / metabolism*
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Bioreactors / microbiology
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Cell Culture Techniques / methods*
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Escherichia coli / genetics*
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Escherichia coli / metabolism*
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Humans
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Lactose / administration & dosage*
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Lactose / metabolism
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Protein Engineering / methods*
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Recombinant Proteins / metabolism
Substances
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B-Cell Activating Factor
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Recombinant Proteins
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Lactose