Inference of DNA sequences from mechanical unzipping: an ideal-case study

Phys Rev Lett. 2006 Mar 31;96(12):128102. doi: 10.1103/PhysRevLett.96.128102. Epub 2006 Mar 30.

Abstract

The performances of Bayesian inference to predict the sequence of DNA molecules from fixed-force unzipping experiments are investigated. We show that the probability of misprediction decreases exponentially with the amount of collected data. The decay rate is calculated as a function of biochemical parameters (binding free energies), the sequence content, the applied force, the elastic properties of a DNA single strand, and time resolution.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriophage lambda / chemistry
  • Bacteriophage lambda / genetics
  • Base Sequence
  • Bayes Theorem
  • Biophysical Phenomena
  • Biophysics
  • DNA / chemistry
  • DNA / genetics*
  • DNA, Viral / chemistry
  • DNA, Viral / genetics
  • Elasticity
  • Models, Statistical
  • Sequence Analysis, DNA / methods*
  • Sequence Analysis, DNA / statistics & numerical data
  • Thermodynamics

Substances

  • DNA, Viral
  • DNA