A preparative isolation and purification of arctigenin and matairesinol from Forsythia koreana by centrifugal partition chromatography

J Sep Sci. 2006 Mar;29(5):656-9. doi: 10.1002/jssc.200500316.

Abstract

Centrifugal partition chromatography was applied to separate arctigenin and matairesinol from Forsythia koreana extract with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (5:5:5:5 v/v). Using this method, arctigenin and matairesinol were successfully separated from partially purified F. koreana extracts in only one step. The purities of isolated compounds were determined to be over 90% by HPLC analysis.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid / methods
  • Countercurrent Distribution / methods*
  • Forsythia / chemistry*
  • Furans / analysis
  • Furans / isolation & purification*
  • Lignans / analysis
  • Lignans / isolation & purification*
  • Molecular Structure
  • Plant Extracts / chemistry
  • Solvents / chemistry

Substances

  • Furans
  • Lignans
  • Plant Extracts
  • Solvents
  • matairesinol
  • arctigenin