PRIMA: peptide robust identification from MS/MS spectra

J Bioinform Comput Biol. 2006 Feb;4(1):125-38. doi: 10.1142/s0219720006001746.

Abstract

In proteomics, tandem mass spectrometry is the key technology for peptide sequencing. However, partially due to the deficiency of peptide identification software, a large portion of the tandem mass spectra are discarded in almost all proteomics centers because they are not interpretable. The problem is more acute with the lower quality data from low end but more popular devices such as the ion trap instruments. In order to deal with the noisy and low quality data, this paper develops a systematic machine learning approach to construct a robust linear scoring function, whose coefficients are determined by a linear programming. A prototype, PRIMA, was implemented. When tested with large benchmarks of varying qualities, PRIMA consistently has higher accuracy than commonly used software MASCOT, SEQUEST and X! Tandem.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Artificial Intelligence
  • Computational Biology
  • Mass Spectrometry / methods*
  • Mass Spectrometry / statistics & numerical data
  • Molecular Sequence Data
  • Peptides / chemistry*
  • Peptides / genetics
  • Programming, Linear
  • Proteomics / statistics & numerical data*
  • Sequence Analysis, Protein / statistics & numerical data
  • Software

Substances

  • Peptides