Quantitative scattering of melanin solutions

Biophys J. 2006 Jun 1;90(11):4137-44. doi: 10.1529/biophysj.105.075713. Epub 2006 Mar 24.

Abstract

The optical scattering coefficient of a dilute, well-solubilized eumelanin solution has been accurately measured as a function of incident wavelength, and found to contribute <6% of the total optical attenuation between 210 and 325 nm. At longer wavelengths (325-800 nm), the scattering was less than the minimum sensitivity of our instrument. This indicates that ultraviolet and visible optical density spectra can be interpreted as true absorption with a high degree of confidence. The scattering coefficient versus wavelength was found to be consistent with Rayleigh theory for a particle radius of 38 +/- 1 nm. Our results shed important light on the role of melanins as photoprotectants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Light
  • Melanins / chemistry*
  • Models, Theoretical*
  • Scattering, Radiation
  • Solutions / chemistry
  • Spectrum Analysis

Substances

  • Melanins
  • Solutions
  • eumelanin