Abstract
Dehydroevodiamine is a major bioactive quinazoline alkaloid isolated from Evodiae Fructus. We investigated the anti-inflammatory properties of dehydroevodiamine in RAW 264.7 murine macrophages. The results indicated that dehydroevodiamine inhibited the expression of LPS-induced iNOS and COX-2 proteins and suppressed also their mRNAs from RT-PCR experiment on RAW 264.7 cells. Furthermore, this compound inhibited the level of LPS-stimulated prostaglandin E2 (PGE2) and LPS-induced nuclear factor-kappa B (NF-kappaB). Therefore, we suggested that the effect of dehydroevodiamine-mediated inhibition of the expression LPS-induced iNOS and COX-2 genes is due to under the suppression of NF-kappaB activation in the transcriptional level.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkaloids / pharmacology*
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Animals
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Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
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Blotting, Western
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Cell Nucleus / drug effects
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Cell Nucleus / enzymology
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Cyclooxygenase 2 / biosynthesis*
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Cyclooxygenase 2 Inhibitors*
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Dinoprostone / biosynthesis
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Electrophoretic Mobility Shift Assay
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Enzyme Induction / drug effects
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Gene Expression Regulation, Enzymologic / drug effects
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Lipopolysaccharides / antagonists & inhibitors*
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Lipopolysaccharides / pharmacology
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Macrophages / drug effects
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Macrophages / enzymology*
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Mice
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NF-kappa B / antagonists & inhibitors*
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NF-kappa B / metabolism
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Neuroprotective Agents / pharmacology
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Nitric Oxide Synthase Type II / biosynthesis*
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RNA / biosynthesis
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RNA / genetics
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Reverse Transcriptase Polymerase Chain Reaction
Substances
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Alkaloids
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Anti-Inflammatory Agents, Non-Steroidal
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Cyclooxygenase 2 Inhibitors
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Lipopolysaccharides
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NF-kappa B
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Neuroprotective Agents
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RNA
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dehydroevodiamine
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Nitric Oxide Synthase Type II
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Cyclooxygenase 2
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Dinoprostone