Abstract
The immunostimulatory oligodeoxynucleotide (ODN) BL07 (5'-GCGTCGGTTTCGGTGCTCAC-3') was identified from the genomic DNA of the probiotic strain Bifidobacterium longum BB536. ODN BL07 stimulated B-lymphocyte proliferation and induced interleukin-12 (IL-12) production in macrophage-like J774.1 cells. ODNs BL07 and BL07S (modified with phosphorothioate backbone) significantly inhibited immunoglobulin E (IgE) production and stimulated interferon-gamma (IFN-gamma) and IL-12 production, but did not affect IL-4 secretion in murine splenic cells of ovalbumin-primed BALB/c mice. These ODNs also significantly inhibited production of IgE in purified murine B cells in the presence of IL-4 and anti-CD40. The results suggest the potential of ODNs BL07 and BL07S in preventing IgE-related immune responses and the possible involvement of ODN BL07 in the antiallergic efficacy of B. longum BB536.
MeSH terms
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Animals
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B-Lymphocytes / cytology
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B-Lymphocytes / immunology
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Base Sequence
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Bifidobacterium / genetics*
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Bifidobacterium / immunology*
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Cell Proliferation
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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DNA, Bacterial / immunology*
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Enzyme-Linked Immunosorbent Assay
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Immunoglobulin E / biosynthesis*
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Immunoglobulin E / immunology
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Immunoglobulin Isotypes / immunology
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Interferon-gamma / immunology
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Interleukin-12 / immunology
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Interleukin-4 / immunology
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Mice
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Mice, Inbred BALB C
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Molecular Sequence Data
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NF-kappa B / immunology
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Oligodeoxyribonucleotides / genetics*
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Oligodeoxyribonucleotides / immunology
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Oligodeoxyribonucleotides / isolation & purification
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Probiotics / pharmacology*
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Sequence Analysis, DNA
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Specific Pathogen-Free Organisms
Substances
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DNA, Bacterial
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Immunoglobulin Isotypes
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NF-kappa B
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Oligodeoxyribonucleotides
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Interleukin-12
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Interleukin-4
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Immunoglobulin E
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Interferon-gamma