Protein engineering of formate dehydrogenase

Biomol Eng. 2006 Jun;23(2-3):89-110. doi: 10.1016/j.bioeng.2006.02.003. Epub 2006 Mar 20.

Abstract

NAD+-dependent formate dehydrogenase (FDH, EC 1.2.1.2) is one of the best enzymes for the purpose of NADH regeneration in dehydrogenase-based synthesis of optically active compounds. Low operational stability and high production cost of native FDHs limit their application in commercial production of chiral compounds. The review summarizes the results on engineering of bacterial and yeast FDHs aimed at improving their chemical and thermal stability, catalytic activity, switch in coenzyme specificity from NAD+ to NADP+ and overexpression in Escherichia coli cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Amino Acid Sequence
  • Bioreactors
  • Coenzymes / genetics
  • Coenzymes / isolation & purification
  • Coenzymes / metabolism
  • Escherichia coli / metabolism
  • Formate Dehydrogenases / genetics
  • Formate Dehydrogenases / isolation & purification
  • Formate Dehydrogenases / metabolism*
  • Models, Molecular
  • Molecular Sequence Data
  • Mutation
  • Protein Engineering*
  • Recombinant Proteins / genetics
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism
  • Sequence Homology, Amino Acid
  • Yeasts / metabolism

Substances

  • Coenzymes
  • Recombinant Proteins
  • Formate Dehydrogenases