[Studies on the preparation and characterization of immobilized neutral protease by carboxymethyl chitosan microsphere]

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2006 Feb;23(1):97-101.
[Article in Chinese]

Abstract

AS1. 398 neutral protease was immobilized onto carboxymethyl chitosan with glutaraldehyde as cross-linking agent. The effects of pH, time of cross-linking, amount of cross-linking agent and the ratio of enzyme to carrier on the activity of the immobilized enzyme were demonstrated, and the optimum immobilization condition of AS1. 398 neutral protease was established. Also studied was the characterization of immobilized enzyme,including pH, temperature, Km and the stability of storage.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / chemistry*
  • Chitosan / analogs & derivatives*
  • Chitosan / pharmacology
  • Endopeptidases / chemistry*
  • Enzymes, Immobilized / chemical synthesis*
  • Enzymes, Immobilized / chemistry
  • Glutaral / pharmacology
  • Microspheres*

Substances

  • Bacterial Proteins
  • Enzymes, Immobilized
  • carboxymethyl-chitosan
  • Chitosan
  • Endopeptidases
  • neutral protease, Bacillus stearothermophilus
  • Glutaral