Pulsed-field gel electrophoresis as a discriminatory typing technique for the biothreat agent burkholderia mallei

Am J Trop Med Hyg. 2006 Mar;74(3):345-7.

Abstract

Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) was used to type 21 laboratory strains of Burkholderia mallei. We demonstrated good resolution by PFGE together with clustering of some geographically related isolates, and confirmed previous observations that B. mallei is clonal as defined by MLST.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Animals
  • Bioterrorism
  • Burkholderia mallei / classification*
  • Burkholderia mallei / genetics
  • Burkholderia mallei / isolation & purification
  • Cluster Analysis
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Electrophoresis, Gel, Pulsed-Field / methods*
  • Glanders / microbiology*
  • Horses
  • Humans
  • Polymerase Chain Reaction / veterinary
  • Serotyping

Substances

  • DNA, Bacterial