Abstract
[reaction: see text] Azinomycins have potential therapeutic value as antitumor agents; however, their biosynthesis is poorly understood. Here, we provide the first demonstration of a protein cell-free system capable of supporting complete in vitro biosynthesis of the antitumor agent azinomycin B. The cell-free system is utilized to probe the cofactor dependence and substrate requirements of the pathway en route to azinomycin.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents / chemical synthesis*
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Antineoplastic Agents / chemistry
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Catalysis
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Intercellular Signaling Peptides and Proteins
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Molecular Structure
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Naphthalenes / chemical synthesis
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Naphthalenes / chemistry
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Peptides / chemical synthesis*
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Peptides / chemistry
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Streptomyces / enzymology*
Substances
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Antineoplastic Agents
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Intercellular Signaling Peptides and Proteins
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Naphthalenes
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Peptides
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azinomycin B