In vitro biosynthesis of the antitumor agent azinomycin B

Org Lett. 2006 Mar 16;8(6):1065-8. doi: 10.1021/ol052987l.

Abstract

[reaction: see text] Azinomycins have potential therapeutic value as antitumor agents; however, their biosynthesis is poorly understood. Here, we provide the first demonstration of a protein cell-free system capable of supporting complete in vitro biosynthesis of the antitumor agent azinomycin B. The cell-free system is utilized to probe the cofactor dependence and substrate requirements of the pathway en route to azinomycin.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / chemical synthesis*
  • Antineoplastic Agents / chemistry
  • Catalysis
  • Intercellular Signaling Peptides and Proteins
  • Molecular Structure
  • Naphthalenes / chemical synthesis
  • Naphthalenes / chemistry
  • Peptides / chemical synthesis*
  • Peptides / chemistry
  • Streptomyces / enzymology*

Substances

  • Antineoplastic Agents
  • Intercellular Signaling Peptides and Proteins
  • Naphthalenes
  • Peptides
  • azinomycin B