Expression and purification of soluble non-fusion vasostatin in Escherichia coli

Protein Pept Lett. 2005 Oct;12(7):659-61. doi: 10.2174/0929866054696163.

Abstract

Vasostatin has previously been expressed in fused form or in inclusion body form in Escherichia coli. Here the protein was expressed in soluble non-fusion form in BL21(DE3)pLysS by IPTG induction. The expression level of vasostatin was about 15% of the total cellular protein. The expressed vasostatin was purified and its biological activity was investigated by an endothelial cell proliferation assay.

MeSH terms

  • Calreticulin / genetics
  • Calreticulin / isolation & purification*
  • Calreticulin / metabolism*
  • Calreticulin / pharmacology
  • Cell Line
  • Cell Proliferation / drug effects
  • Endothelial Cells / drug effects
  • Escherichia coli / genetics*
  • Gene Expression / genetics*
  • Peptide Fragments / genetics
  • Peptide Fragments / isolation & purification*
  • Peptide Fragments / metabolism*
  • Peptide Fragments / pharmacology
  • Recombinant Proteins / genetics
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism
  • Recombinant Proteins / pharmacology
  • Solubility

Substances

  • Calreticulin
  • Peptide Fragments
  • Recombinant Proteins
  • vasostatin