Abstract
Vasostatin has previously been expressed in fused form or in inclusion body form in Escherichia coli. Here the protein was expressed in soluble non-fusion form in BL21(DE3)pLysS by IPTG induction. The expression level of vasostatin was about 15% of the total cellular protein. The expressed vasostatin was purified and its biological activity was investigated by an endothelial cell proliferation assay.
MeSH terms
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Calreticulin / genetics
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Calreticulin / isolation & purification*
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Calreticulin / metabolism*
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Calreticulin / pharmacology
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Cell Line
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Cell Proliferation / drug effects
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Endothelial Cells / drug effects
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Escherichia coli / genetics*
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Gene Expression / genetics*
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Peptide Fragments / genetics
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Peptide Fragments / isolation & purification*
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Peptide Fragments / metabolism*
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Peptide Fragments / pharmacology
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Recombinant Proteins / genetics
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / metabolism
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Recombinant Proteins / pharmacology
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Solubility
Substances
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Calreticulin
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Peptide Fragments
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Recombinant Proteins
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vasostatin