Usefulness of eschar PCR for diagnosis of scrub typhus

J Clin Microbiol. 2006 Mar;44(3):1169-71. doi: 10.1128/JCM.44.3.1169-1171.2006.

Abstract

We report here on the case of a child who was infected with scrub typhus, and we made the diagnosis according to the serology and by performing PCRs on the child's eschar. The patient was treated with azithromycin, and he did not experience any complications. Performing nested PCR on the eschar might be both a rapid diagnostic test for scrub typhus in the early acute stage and a differential test as to whether or not a scab is a scrub typhus eschar.

Publication types

  • Case Reports
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Bacterial / genetics
  • Bacterial Proteins / genetics
  • Base Sequence
  • Child
  • DNA Primers / genetics
  • DNA, Bacterial / genetics
  • DNA, Bacterial / isolation & purification
  • Humans
  • Male
  • Membrane Proteins / genetics
  • Orientia tsutsugamushi / genetics
  • Orientia tsutsugamushi / isolation & purification
  • Polymerase Chain Reaction / methods*
  • Scrub Typhus / diagnosis*
  • Scrub Typhus / microbiology
  • Skin / microbiology

Substances

  • Antigens, Bacterial
  • Bacterial Proteins
  • DNA Primers
  • DNA, Bacterial
  • Membrane Proteins
  • Sta56 antigen, Rickettsia tsutsugamushi