Abstract
The Sendai virus V protein, which is a fusion of the P and V ORFs of the P gene, was characterized with antisera to a portion of the V ORF and compared to the P protein. The only property found in common with P is that V is also highly phosphorylated, and this is so even when these proteins are expressed independently of the other viral proteins. Otherwise, V was not found in virions, was not strongly associated with viral nucleocapsids like P, and anti-V had no effect on viral RNA synthesis in vitro under conditions where anti-P was highly inhibitory. The available evidence suggests that V may play a role in RNA synthesis, but it is not an essential one like that of the P protein.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies
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Antigen-Antibody Complex
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Cell Line
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Electrophoresis, Polyacrylamide Gel
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Fluorescent Antibody Technique
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Genes, Viral
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Molecular Sequence Data
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Molecular Weight
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Open Reading Frames
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Parainfluenza Virus 1, Human / genetics*
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Peptides / chemical synthesis
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Peptides / immunology
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Phosphorylation
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RNA, Messenger / genetics
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RNA, Viral / genetics
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Ribonucleoproteins / genetics
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Ribonucleoproteins / isolation & purification
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Viral Proteins / analysis*
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Viral Proteins / genetics
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Viral Proteins / immunology
Substances
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Antibodies
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Antigen-Antibody Complex
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Peptides
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RNA, Messenger
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RNA, Viral
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Ribonucleoproteins
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Viral Proteins