Abstract
N-Acetyl-gamma-glutamyl-phosphate reductase (AGPR) catalyzes the third step in an eight-step arginine-biosynthetic pathway that starts with glutamate. This enzyme converts N-acetyl-gamma-glutamyl phosphate to N-acetylglutamate-gamma-semialdehyde by an NADPH-dependent reductive dephosphorylation. AGPR from Oryza sativa (OsAGPR) was expressed in Escherichia coli at 291 K as a soluble fusion protein with an upstream thioredoxin-hexahistidine [Trx-(His)6] extension. OsAGPR(Ala50-Pro366) was purified and crystals were obtained using the sitting-drop vapour-diffusion method at 293 K and diffract X-rays to at least 1.8 A resolution. They belong to the hexagonal space group P6(1), with unit-cell parameters a = 86.11, c = 316.3 A.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aldehyde Oxidoreductases / chemistry*
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Amino Acids / chemistry
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Cloning, Molecular
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Crystallization
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Crystallography, X-Ray
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Diffusion
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Escherichia coli / metabolism
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Glutamates / chemistry
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Histidine / chemistry
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Humans
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NADP / chemistry
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Oligopeptides / chemistry
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Oryza / enzymology*
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Protein Conformation
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Recombinant Fusion Proteins / chemistry
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Recombinant Proteins / chemistry
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Temperature
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Thioredoxins / chemistry
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X-Ray Diffraction
Substances
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Amino Acids
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Glutamates
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His-His-His-His-His-His
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Oligopeptides
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Recombinant Fusion Proteins
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Recombinant Proteins
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Histidine
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Thioredoxins
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NADP
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Aldehyde Oxidoreductases
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N-acetyl-gamma-glutamyl-phosphate reductase