Crystallization and preliminary crystallographic analysis of a family 43 beta-D-xylosidase from Geobacillus stearothermophilus T-6

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Dec 1;61(Pt 12):1054-7. doi: 10.1107/S1744309105036262. Epub 2005 Nov 12.

Abstract

Beta-D-Xylosidases (EC 3.2.1.37) are hemicellulases that cleave single xylose units from the nonreducing end of xylooligomers. In this study, the crystallization and preliminary X-ray analysis of a beta-D-xylosidase from Geobacillus stearothermophilus T-6 (XynB3), a family 43 glycoside hydrolase, is described. XynB3 is a 535-amino-acid protein with a calculated molecular weight of 61 891 Da. Purified recombinant native and catalytic inactive mutant proteins were crystallized and cocrystallized with xylobiose in two different space groups, P2(1)2(1)2 (unit-cell parameters a = 98.32, b = 99.36, c = 258.64 A) and P4(1)2(1)2 (or the enantiomorphic space group P4(3)2(1)2; unit-cell parameters a = b = 140.15, c = 233.11 A), depending on the detergent. Transferring crystals to cryoconditions required a very careful protocol. Orthorhombic crystals diffract to 2.5 A and tetragonal crystals to 2.2 A.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Buffers
  • Catalysis
  • Cryopreservation
  • Crystallization
  • Crystallography, X-Ray / methods*
  • Disaccharides / chemistry
  • Escherichia coli / metabolism
  • Geobacillus stearothermophilus / enzymology*
  • Glycoside Hydrolases / chemistry
  • Molecular Weight
  • Mutation
  • Protein Conformation
  • Recombinant Proteins / chemistry
  • X-Ray Diffraction
  • Xylosidases / chemistry*

Substances

  • Buffers
  • Disaccharides
  • Recombinant Proteins
  • Glycoside Hydrolases
  • Xylosidases
  • exo-1,4-beta-D-xylosidase
  • xylobiose