Malaria constitutes a major public health problem on Mayotte Island, with the major vector being Anopheles gambiae s.s. The seeming disappearance of An. funestus, the 2nd vector on the island, was observed 15 years ago. Now, the presence of An. funestus during the biting collections performed in November and December 2004 confirms the re-emergence of this vector, which plays an important role in malaria transmission with An. gambiae.