A simple high performance liquid chromatography assay for monitoring plasma concentrations of tipranavir in HIV infected patients

E Dailly; V Reliquet; C Victorri-Vigneau; F Raffi; P Jolliet

doi:10.1016/j.jchromb.2006.02.004

A simple high performance liquid chromatography assay for monitoring plasma concentrations of tipranavir in HIV infected patients

J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Mar 7;832(2):317-20. doi: 10.1016/j.jchromb.2006.02.004. Epub 2006 Feb 23.

Authors

E Dailly¹, V Reliquet, C Victorri-Vigneau, F Raffi, P Jolliet

Affiliation

¹ Clinical Pharmacology Department, Hôtel Dieu, Nantes, France. eric.dailly@chu-nantes.fr

PMID: 16497564
DOI: 10.1016/j.jchromb.2006.02.004

Abstract

A simple HPLC assay to determine plasma concentration of tipranavir is presented. A liquid/liquid extraction of the drugs in ethyl acetate/hexane from 250 microL of plasma is followed by a reversed phase isocratic HPLC assay with UV detection at 205 nm. The imprecision and inaccuracy are lower than 10%, the low limit of quantitation is 0.4 mg/L. Thus, this method can be used for therapeutic drug monitoring of tipranavir in HIV infected patients.

MeSH terms

Chromatography, High Pressure Liquid / methods*
HIV Infections / blood
HIV Infections / drug therapy*
Humans
Pyridines / blood*
Pyridines / therapeutic use
Pyrones / blood*
Pyrones / therapeutic use
Reproducibility of Results
Reverse Transcriptase Inhibitors / blood*
Reverse Transcriptase Inhibitors / therapeutic use
Sensitivity and Specificity
Spectrophotometry, Ultraviolet
Sulfonamides

Substances

Pyridines
Pyrones
Reverse Transcriptase Inhibitors
Sulfonamides
tipranavir