RNA specific molecules: cytotoxic plant alkaloid palmatine binds strongly to poly(A)

Prabal Giri; Maidul Hossain; Gopinatha Suresh Kumar

doi:10.1016/j.bmcl.2006.01.124

RNA specific molecules: cytotoxic plant alkaloid palmatine binds strongly to poly(A)

Bioorg Med Chem Lett. 2006 May 1;16(9):2364-8. doi: 10.1016/j.bmcl.2006.01.124. Epub 2006 Feb 23.

Authors

Prabal Giri¹, Maidul Hossain, Gopinatha Suresh Kumar

Affiliation

¹ Biophysical Chemistry Laboratory, Indian Institute of Chemical Biology, Kolkata 700 032, India.

PMID: 16497501
DOI: 10.1016/j.bmcl.2006.01.124

Abstract

The cytotoxic plant alkaloid palmatine was found to bind strongly by partial intercalation to single stranded poly(A) structure with binding affinity (Ka) of (8.36+/-0.26) x 10(5) M(-1). The binding of palmatine was characterized to be exothermic and enthalpy driven with one palmatine for every two adenine residues. On the other hand, the binding to the double stranded poly(A) has been found to be significantly weak. This study identifies poly(A) as a potential bio-target for the alkaloid palmatine and its use as a lead compound in antitumor drug screening.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antineoplastic Agents / chemistry*
Berberine Alkaloids / chemistry*
Circular Dichroism
Drug Evaluation, Preclinical
Hydrogen Bonding
Molecular Structure
Poly A / chemistry*
RNA / chemistry*
Thermodynamics

Substances

Antineoplastic Agents
Berberine Alkaloids
Poly A
RNA
palmatine