Alcohol-inducible gene expression in transgenic Populus

Plant Cell Rep. 2006 Jul;25(7):660-7. doi: 10.1007/s00299-005-0112-3. Epub 2006 Feb 23.

Abstract

We tested the efficiency and optimized the conditions for controlled alcohol-inducible transgene expression in Populus using gus as a reporter gene. Specificity of induction, efficiency in different organs, effect of three chemical inducers, and induction methods were tested using up to 10 independent transgenic events generated in two different Populus genotypes. The optimal inducer concentration and the duration of induction period were determined in dose-response and in time-course experiments. Under in vitro conditions, beta-glucuronidase (GUS) induction was efficient both in the aerial parts and in the roots of regenerated plantlets. Among the chemical inducers tested, ethanol was the most effective activator with no apparent phytotoxicity when concentrations were at or below 2%. After 5 days of treatment, fluorometrically-determined the GUS activity could be detected when inducing with ethanol at concentrations as low as 0.5%. Prolonged induction by ethanol vapors significantly increased the GUS activity in leaves from both the tissue culture plants and greenhouse-grown plants.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Acetaldehyde / pharmacology
  • Butanones / pharmacology
  • Dose-Response Relationship, Drug
  • Ethanol / administration & dosage
  • Ethanol / pharmacology*
  • Gene Expression Regulation, Plant / drug effects*
  • Genes, Plant / genetics*
  • Genes, Reporter / genetics
  • Plant Leaves / drug effects
  • Plant Leaves / genetics
  • Plant Roots
  • Plants, Genetically Modified
  • Populus / drug effects*
  • Populus / genetics*
  • Time Factors
  • Volatilization

Substances

  • Butanones
  • Ethanol
  • methylethyl ketone
  • Acetaldehyde