Auxin is a multifactorial phytohormone that is required for cell division. Fine gradients determine points of developmental change in time and space. It is associated intimately with the axiality of plant growth, and increasing doses lead to cell expansion or inhibition of cell expansion in different tissues. From embryonic patterning to fruit dehiscence every plant process has some involvement with auxin as a hormonal signal, including responses to wounding. Moreover, synthetic auxins have widespread uses as agrochemicals, particularly as selective herbicides. Despite the importance of auxin as a plant signal the pathways of its biosynthesis are still not clear. Much more is known about auxin perception and the mechanisms through which gene transcription is regulated. One receptor has been identified, and protein crystallography data has explained its auxin-binding capacity, but this is likely to control only a subset of auxin-mediated responses. Little is known of the signal transduction intermediates. A second receptor has been nominated and may be involved in controlling auxin-mediated gene transcription. A complex set of proteins comprising signalosome and proteasome contribute to the regulation of sets of transcription factors to confer regulation by derepression. A set of auxin transport proteins has been described with associated regulatory interactors, and these account for polar auxin flow and the control of auxin movements across cells, tissues, and around the plant. The gradients these transport systems build regulate the responses of growth and differentiation, including the plant's response to gravity. These areas are described and discussed by relating the physiology of the whole plant to the details of genetic and protein activities.