Isolation and characterization of a novel thraustochytrid-like microorganism that efficiently produces docosahexaenoic acid

Zakia Perveen; Hitomi Ando; Akio Ueno; Yukiya Ito; Yusuke Yamamoto; Yohko Yamada; Tomoko Takagi; Takako Kaneko; Kazuhiro Kogame; Hidetoshi Okuyama

doi:10.1007/s10529-005-5335-4

Isolation and characterization of a novel thraustochytrid-like microorganism that efficiently produces docosahexaenoic acid

Biotechnol Lett. 2006 Feb;28(3):197-202. doi: 10.1007/s10529-005-5335-4.

Authors

Zakia Perveen¹, Hitomi Ando, Akio Ueno, Yukiya Ito, Yusuke Yamamoto, Yohko Yamada, Tomoko Takagi, Takako Kaneko, Kazuhiro Kogame, Hidetoshi Okuyama

Affiliation

¹ Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo, Kita-ku, 060-0810, Japan.

PMID: 16489498
DOI: 10.1007/s10529-005-5335-4

Abstract

A thraustochytrid-like microorganism (strain 12B) was isolated from the mangrove area of Okinawa, Japan. On the basis of its ectoplasmic net structure and biflagellate zoospores we determined strain 12B to be a novel member of the phylum Labyrinthulomycota in the kingdom Protoctista. When grown on glucose/seawater at 28 degrees C, it had a lipid content of 58% with docosahexaenoic acid (DHA; 22:6 n-3) at 43% of the total fatty acids. It had a growth rate of 0.38 h(-1). The DHA production rate of 2.8 +/- 0.7 g l(-1) day(-1) is the highest value reported for any microorganism.

MeSH terms

Docosahexaenoic Acids / metabolism*
Fungi / metabolism*
Lipid Metabolism*
Magnoliopsida / microbiology*
Plant Leaves / microbiology*

Substances

Docosahexaenoic Acids