MYB transcription factors play important roles in many plant developmental processes and various defense responses. Two groups of MYB transcription factors were found to share a W/Y-MDDIW motif. This motif alone shows no trans-activation activity in yeast, however, it enhances the trans-activation activity of the neighbor regions greatly. We further show that all the genes in the group 1, including the previously reported genes AtMYB21, PsMYB26, AmMYB305, and AmMYB340, are predominantly expressed in flowers. Furthermore, we found that these two groups of MYB transcription factor genes might be regulated by light, and probably preferentially expressed in the darkness, suggesting that they may play roles in the light signaling pathway.