Abstract
A dual-antigen enzyme-linked immunosorbent assay specific for human immunodeficiency virus type 2 (HIV-2) envelope proteins, ELISA-HIV2, was developed with two new recombinant polypeptides, rpC2-C3 and rgp36, derived from the HIV-2 envelope. The diagnostic performance was determined with HIV-2, HIV-1, and HIV-1/2 samples. Both polypeptides showed 100% specificity. Clinical sensitivity was 100% for rgp36 and 93.4% for rpC2-C3. ELISA-HIV2 may be used for the specific diagnosis and confirmation of HIV-2 infection.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Enzyme-Linked Immunosorbent Assay
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Gene Products, env / genetics
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Gene Products, env / immunology*
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HIV Antibodies / blood*
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HIV Antigens / genetics
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HIV Antigens / immunology*
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HIV Infections / diagnosis*
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HIV Infections / virology
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HIV-2 / immunology*
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Humans
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Protein Precursors / genetics
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Protein Precursors / immunology*
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Recombinant Proteins / genetics
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Recombinant Proteins / immunology
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Sensitivity and Specificity
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env Gene Products, Human Immunodeficiency Virus
Substances
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Gene Products, env
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HIV Antibodies
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HIV Antigens
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Protein Precursors
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Recombinant Proteins
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env Gene Products, Human Immunodeficiency Virus
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env protein gp36, Human immunodeficiency virus
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gp125 envelope protein, Human immunodeficiency virus 2