Use of a new dual-antigen enzyme-linked immunosorbent assay to detect and characterize the human antibody response to the human immunodeficiency virus type 2 envelope gp125 and gp36 glycoproteins

J Clin Microbiol. 2006 Feb;44(2):607-11. doi: 10.1128/JCM.44.2.607-611.2006.

Abstract

A dual-antigen enzyme-linked immunosorbent assay specific for human immunodeficiency virus type 2 (HIV-2) envelope proteins, ELISA-HIV2, was developed with two new recombinant polypeptides, rpC2-C3 and rgp36, derived from the HIV-2 envelope. The diagnostic performance was determined with HIV-2, HIV-1, and HIV-1/2 samples. Both polypeptides showed 100% specificity. Clinical sensitivity was 100% for rgp36 and 93.4% for rpC2-C3. ELISA-HIV2 may be used for the specific diagnosis and confirmation of HIV-2 infection.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Enzyme-Linked Immunosorbent Assay
  • Gene Products, env / genetics
  • Gene Products, env / immunology*
  • HIV Antibodies / blood*
  • HIV Antigens / genetics
  • HIV Antigens / immunology*
  • HIV Infections / diagnosis*
  • HIV Infections / virology
  • HIV-2 / immunology*
  • Humans
  • Protein Precursors / genetics
  • Protein Precursors / immunology*
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology
  • Sensitivity and Specificity
  • env Gene Products, Human Immunodeficiency Virus

Substances

  • Gene Products, env
  • HIV Antibodies
  • HIV Antigens
  • Protein Precursors
  • Recombinant Proteins
  • env Gene Products, Human Immunodeficiency Virus
  • env protein gp36, Human immunodeficiency virus
  • gp125 envelope protein, Human immunodeficiency virus 2