Complete 1H and 13C NMR assignment of digeneaside, a low-molecular-mass carbohydrate produced by red seaweeds

Sérgio D Ascêncio; Alexandre Orsato; Robson A França; M Eugênia R Duarte; Miguel D Noseda

doi:10.1016/j.carres.2006.01.002

Complete 1H and 13C NMR assignment of digeneaside, a low-molecular-mass carbohydrate produced by red seaweeds

Carbohydr Res. 2006 Apr 10;341(5):677-82. doi: 10.1016/j.carres.2006.01.002. Epub 2006 Jan 26.

Authors

Sérgio D Ascêncio¹, Alexandre Orsato, Robson A França, M Eugênia R Duarte, Miguel D Noseda

Affiliation

¹ Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, PO Box 19046, CEP 81531-990, Curitiba, Paraná, Brazil.

PMID: 16442514
DOI: 10.1016/j.carres.2006.01.002

Abstract

Digeneaside (alpha-D-mannopyranosyl-(1-->2)-D-glycerate) was extracted from the red algae, Bostrychia binderii, and purified by adsorption and gel-filtration chromatography. HPLC and ESI-MS techniques were used to follow purification steps and characterize digeneaside. NMR spectroscopy experiments (1D 1H, 13C, DEPT and 2D HMQC, COSY and TOCSY) were used to fully assign the 1H and 13C spectra.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbohydrate Sequence
Carbon Isotopes
Chromatography, High Pressure Liquid
Glyceric Acids / chemistry
Lactic Acid / analogs & derivatives*
Lactic Acid / chemistry
Mannose / chemistry
Mannosides / chemistry*
Molecular Structure
Nuclear Magnetic Resonance, Biomolecular
Seaweed / chemistry*
Seaweed / metabolism
Spectrometry, Mass, Electrospray Ionization

Substances

Carbon Isotopes
Glyceric Acids
Mannosides
digeneaside
Lactic Acid
glyceric acid
Mannose