Immune responses induced in cattle by vaccination with a recombinant adenovirus expressing Mycobacterial antigen 85A and Mycobacterium bovis BCG

Infect Immun. 2006 Feb;74(2):1416-8. doi: 10.1128/IAI.74.2.1416-1418.2006.

Abstract

Cattle were vaccinated with an adenovirus expressing the mycobacterial antigen 85A (rAd85A), with Mycobacterium bovis BCG followed by rAd85A heterologous boosting, or with rAd85A followed by BCG boosting. BCG/rAd85A resulted in the highest direct gamma interferon responses. Cultured enzyme-linked immunospot assay analysis demonstrated that memory responses were induced by all three protocols but were strongest after BCG/rAd85A and rAd85A/BCG vaccination.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviridae / genetics*
  • Adenoviridae / immunology
  • Animals
  • Antigens, Bacterial / administration & dosage*
  • Antigens, Bacterial / genetics
  • Antigens, Bacterial / immunology*
  • BCG Vaccine / administration & dosage*
  • BCG Vaccine / immunology*
  • Cattle
  • Immunization Schedule
  • Immunization, Secondary
  • Interferon-gamma / biosynthesis*
  • Leukocytes, Mononuclear / immunology
  • Lymphocyte Activation
  • Mycobacterium bovis / immunology*
  • Tuberculosis, Bovine / immunology
  • Tuberculosis, Bovine / prevention & control*
  • Vaccination

Substances

  • Antigens, Bacterial
  • BCG Vaccine
  • antigen 85, Mycobacterium bovis
  • Interferon-gamma